Outcome:
The student completing the course will:
1. Know the main branches of synthetic biology
2. Will learn potential drawbacks and ethical issues raised by synthetic biology development
3. Know different molecular cloning techniques, including Golden Gate assembly and digest/ligate cloning
4. Be able to perform PCR, DNA gel electrophoresis, Bacterial and yeast transformations, DNA extraction from bacterial cells.
5. Learn about different sequencing methods and how to analyze the results of Sanger sequencing
6. Perform carotene and violacein extraction from S. Cerevisiae cells
7. Learn the basics of HPLC and TLC, perform TLC and analyze the results.
Teachers:
Ilona Faustova - Associate Professor of Molecular Biology
Artemi Maljavin - Junior Research Fellow in Molecular Systems Biology
Content:
Topic |
Credit |
Lectors |
1. 1. Molecular cloning basics: amplification of DNA of interest (primer design, PCR), assembly of plasmids (digest/ligate, Golden Gate)
2. Metabolic Engineering
3. Analytical Chromagraphy (HPLC, TLC) |
52 |
Ilona Faustova, Artemi Maljavin |
Location info:
Tartu linn Institute of Bioengineering
Nooruse 1-519
Tartu
Learning environment:
Studies and teaching takes place in appropriate classrooms, which have the required teaching equipment and meet the health and safety requirements.
Schedule and further information:
Monday, 29 July
Introduction to summer school: Schedule, goals, learning outcomes.
Requirements to complete:
Participation in all practical sessions.
Outcome method:
non-differentiated (pass, fail, not present)
Grading method:
Active participation in practical sessions
Grading criteria:
Active participation in all practical sessions
Document to be issued:
Certificate of completion/Certificate of attendance
Registration deadline:
12.07.2024
Additional information:
Artemi Maljavin, artemi.maljavin@ut.ee, +372 +37256665967
Program code:
LTBI.TK.001